1.
Morphological Abnormalities Of Spermatozoa In Pure Bred Friesian, Sahiwal Bulls And Friesian X Sahiwal
by Ahsan Rashad, M | Prof. Dr. Rashid Ahmad Ch | Dr. Imtiaz Hussain Khan | Dr. M. Akram | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Of the 15 normal semen producing breeding bulls, five bulls from each of pure bred Sahiwal, Friesian and the cross-breds (Friesian X Sahiwal) of the same age group were selected. These bulls were maintained at Semen Production Unit, Qadirabad (Sahiwal) under similar conditions of housing, feeding and management. Two consecutive ejaculates once a week were collected. A total of 395 ejaculates were evaluated for various seminal characteristics, extended, processed and frozen during August, September and October, 1990. During these three months semen parameters like ejaculate volume, mass motility, percent motility and the doses produced per ejaculate declined with increase of ambient temperature and humidity.
The second ejaculate (195) at each collection and for each bull was always taken for studying the sperm morphology. Two slides from each semen sample were prepared in 10% solution of Nigrosin stain. A total of 200 sperm cells were counted in each slide and average values for various morphological abnormalities of spermatozoa in each breed were studied. The sperm cell abnormalities were accounted for head (abnormal, detached), neck (poor attachment, abaxial attachment and fractured neck), thickened mid-piece, protoplasmic droplet (proximal, distal droplet) and tail (detached, coiled- tail-droplet-enclosed, coiled tail).
The mean values of different breeds so obtained for different abnormalities during various months were analysed. The average values for head abnormalities for three breeds were 9.69 ± 1.88, 5.12 ± 060 and 1.56 ± 0.14% in Friesian, cross-bred and Sahiwal, respectively. These abnormalities were highest during August and September in Friesian bulls and lowest in Sahiwal bulls during October.
The neck abnormalities were highest in cross-breds (4.44 ± 0.57%) and lowest in Sahiwal (1.67 ± 0.14%). However, the neck abnormalities were statistically similar during the months of August, September and October. Similar trend of mid-piece abnormality was observed between various breeds and months. Likewise the values of protoplasmic droplets were highest in cross breds (14.12 ± 1.19%) and lowest in Sahiwal (2.36 ± 0.30%). The tail abnormalities were the highest in cross-breds (3.47 ± 0.45%) and lowest in Sahiwal (0.87 ± 0.12%). Among various months the tail abnormalities, were statistically similar (P> 0.05).
The total values of different sperm abnormalities wçre highest in cross-breds during August (30.93 ± 3.11%) and comparatively lower during September and October (28.66 ± 2.28 and 26.11 ± 2.63%), respectively.
Except head abnormalities, all the other abnormalities of spermatozoa were statistically significant among breeds.
Availability: Items available for loan: UVAS Library [Call number: 0206,T] (1).
2.
Study On Various Seminal Characteristics Affected With Aging Phenomenon In Male Nili-Ravi Buffalo
by Mohsin Raza Naqvi, M | Prof. Dr. Rashid Ahmad Chaudhary | Dr. Nisar | Dr. Tayyab Rahil | Faculty of Veterinary Sciences.
Material type: ; Format:
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not fiction
Publisher: 1991Dissertation note: The main aim of the present study was to observe the reproductive performance of Holstein-Friesian and Jersey cows, imported from USA, being maintained under the sub-tropical environmental conditions of the Punjab, and then to compare it with their respective local-born progenies. The influence of environmental factors on some parameters of reproductive performance had been studied in order to estimate the level of their adoptability towards local climatic conditions. The reproductive records of the normal breeding local-born Holstein-Friesian, imported Holstein-Friesian, local-born Jersey and imported Jersey cows were studied during 1985-1991. The outcome of the present study in respect of the following parameters of reproductive performance are summarized as below.
1) Local-born Holstein-Friesian and local-born Jersey heifers conceived for the first time at the age of 502.93±11.71 and 496.37±6.64 days. This shows that Holstein-Friesian heifers took 7 days more for their first conception, but this difference was found to be non-significant.
2) The average age at first calving for local-born Holstein- Friesian and Jersey heifers was 787.38±13.61 and 771.48±7.88 days respectively, while their respective dams calved in USA for the first time at the age of 822.46±10.63 and 800.51±8.43 days correspondingly. The data indicate that local-born Holstein-Friesian and Jersey heifers calved for the first time 35 and 29 days earlier than their dams.
3) The average number of services required per conception for local-born Holstein-Friesian, imported Holstein-Friesian, local- born Jersey and imported Jersey were 1.76±0.100, 1.87±0.089, 1.83±0.069 and 1.88±0.072 respectively, whereas the difference among the groups was found to be non-significant. Although the number of services required per conception increased with increasing parity but the difference was non-significant.
4) The service period for local-born Holstein-Friesian, imported Holstein-Friesian, local-born Jersey and imported Jersey averaged 161.48±14.45, 132.92±511, 100.99±4.63 and 107.03±2.77 days respectively. The local-born and imported Jersey cows conceived 60 and 26 days earlier after calving than their contemporary local-born and imported Holstein cows. Effect of season of calving on subsequent service period was not significant.
5) The average gestation periods of 278.65±0.78, 279.92±0.63, 279.26±0.51 and 277.49±0.36 days were found in local-born Hol- stein-Friesian, imported Holstein-Friesian, local-born Jersey and imported Jersey cows respectively. The data shows that imported Jersey cows carried their calves 1.16, 2.43 and 1.77 days less than the local-born Holstein-Friesian, imported Holstein-Friesian and local-born Jersey correspondingly. Moreover in all the genetic groups except local-born Holstein-Friesian, cows carrying male calves had longer gestation length than those carrying female calves.
6) The calving interval for local-born Holstein-Friesian, imported Holstein-Friesian, local-born Jersey and imported Jersey averaged 443.77±18.67, 415.66±5.09, 380.93±6.36 and 387.18±3.0 days respectively. The Jerseys showed significantly (P < 0.01) shorter calving interval than the Holsteins. The shortet calving interval in all the groups was seen after Winter calvings. Minimum calvings in each group occurred during Summer. The maximum calvings in local-born Holstein-Friesian and imported Holstein- Friesian cows were during winter; whereas in local-born Jersey and imported Jersey these were observed during Spring and winter in order of merit.
7) The 305-day milk production averaged 3643.29±121.26, 4114.40±115.96, 2194.52±57.25 and 3150.48±46.16 litres for local- born Holstein-Friesian, imported Holstein-Friesian, local-born Jersey and imported Jersey cows respectively. The results indicate that imported Holstein-Friesian and Jersey cows produced -significantly more milk than their respective local-born progenies.
8) The average dry period for local-born Holstein-Friesian, imported Holstein-Friesian, local-born Jersey and imported Jersey cows was 192.71±16.14, 191.07±13.11, 157.91±9.70 and 133.30±5.77 days respectively. The dry period in the local-born Holstein- Friesian and imported Holstein-Friesian was significantly (P < 0.05) higher than the local-born and imported Jersey cows.
The findings of this study regarding most of reproductive traits indicate that both local-born and imported Jersey performed better than Holstein-Friesian cows under sub-tropical climatic conditions of Punjab. However local-born groups produced less 305 days milk than their imported dams. The production potential in the exotic herd can be increased by breeding the cows with semen of genetically superior sires, through intensive management and with more effective disease control programme.
Availability: Items available for loan: UVAS Library [Call number: 0314,T] (1).
3.
Studies On Uterine Involution And Ovarian Activity In Postpartum Nili-Ravi Buffaloes Given Prostaglandin F2 Alpha Analogue
by Farrukh Nazir | Prof. Dr. Rashid Ahmad Chaudhary | Dr. Mian Nisar Ahmad | Dr. Tayyab | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1993Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0324,T] (1).
4.
Antiseperim Antibodies: A Plausible Cause Of Repeat Breeding In Nili-Ravi Buffaloes
by Amir Saeed, M | Prof. Dr. Muhammad Aleem Bhatti | Prof. Dr. Rashid Ahmad Chaudhary | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: The present study was designed with the assumption that high titres of antisperm antibodies in repeat breeders might be a contributing factor for conception failure in such animals. For this purpose, 32 buffaloes/buffalo heifers were divided into four equal groups. From the normal cyclic and repeat breeder buffaloes, 48 serum samples were collected at 0, 1, and 24 hours after insemination, respectively. Oestrus mucus was also collected from these animals. A total of 16 serum samples were collected from virgin heifers and pregnant buffaloes. The serum and mucus samples were subjected to simple agglutination test, sperm quantitative penetration test and sperm cervical mucus contact test. High antibody titres were recorded in the serum and oestrus mucus of repeat breeders. Moreover, it was observed that lesser number of spermatozoa penetrated through a definite distance in cervical mucus of repeat breeder buffaloes as compared to cervical mucus of normal cyclic animals. Similarly higher number of spermatozoa exhibited shaking movement over a period of time in cervical mucus of repeat breeders, which indicates the presence of spermagglutinins.
Thus, it can be concluded that antisperm antibodies may be responsible for conception failure in repeat breeder buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 0383,T] (1).
5.
Induction Of Oestrus In Non Cycling Nili Ravi Buffalo Heifers
by Hafeez Khan, A | Dr. Kazim Raza Chohan | Prof. Dr. Rashid Ahmad Chaudhry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1994Dissertation note: Twenty one non-cycling Nili-Ravi buffalo hcifers with mean (± S.E) age of 31.71 ± 1.32 months and body weight of 417.61 ± 11.26 Kg were randomly divided into three equal groups. Animals in group A were injected 700 IU PMSG intramuscularly. Whereas, animals in group B (n=7) were treated with 1400 IU of PMSG. Animals in group C (n7) were administered 2 CC normal saline, which served as controls. Animals in oestrus were naturally bred with progeny tested bulls. Blood samples were collected before treatment, at oestrus and 10 days after oestrus for plasma progesterone estimation. Rectal palpations were done before treatment, at oestrus, 10 days after oestrus for ovarian structures and on day 60 post breeding for pregnancy diagnosis.
All the buffalo heifers in group A and B exhibited oestrus following treatment with 700 and 1400 IU of PMSG. Three animals from group C (42.85%) showed oestrus. The time from treatment to the onset of oestrus differed non significantly and was 6.14 ± 0.56, 6.00 ± 0.45 and 6.66 ± 0.47 days for groups A, B and C, respectively. No difference was observed towards expression of oestrus symptoms with varying doses of PMSG. The behavioural symptoms and physical changes i.e. frequent urination, acceptance to male, vulvalar sniffing of other females or allowing others to sniff, mucus discharge, vulvalar swelling and uterine tone were found indicative of oestrus in experimental heifers. The duration of oestrus also differed non significantly among the groups. This duration was 22.00 ± 2.01; 27.71 ± 1 .67 and 19.66 ± 0.98 hours for groups A, B and C, respectively.
Three animals from group A (n=7) conceived at induced oestrus. Whereas, no heifer from group B setteled at induced oestrus. Four animals showed next oestrus and two of them conceived. Three animals from group C exhibited oestrus and one of them conceived, rest of the animals remained non cyclic. The overall conception rates were 42.85, 28.57 and 14.28 percent for groups A, B and C, respectively.
Progesterone levels were found representative of ovarian activity in all the groups. Basal levels (> 0.5 ng/ml) of plasma progesterone were observed with smooth ovaries. Higher values for plasma progesterone ranging between 0.95 ± 0.02 to 5.68 ± 0.22 ng/ml were observed in cycling and pregnant animals reprsenting functional corpus lutuem.
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6.
Comparison Of Two Methods Of Estrus Synchronization In Sahiwal Cows
by Dawar Hameed Mughal | Prof. Dr. Muhammad Aleem Bhatti | Dr. Kazim Raza Chohan | Prof. Dr | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1995Dissertation note: A total of 24 Sahiwal cows were selected for this study and randomly divided into two groups viz A and
B. Group A cows were injected two injections of prostaglandin F2 intramuscularly without palpation 11 days apart, while the other group involved the veterinarian skill for detection of palpable corpus luteum and only single injection of prostaglandin was given in all the cows. The cows were artificially inseminated on the basis of standing heat 12 hours after the detection of heat. Estrus detection was made by teasing, behavioural symptoms, physical changes and rectal palpation. 91.66% and 83.33% cows from A and B groups respectively responded to the treatment. This response was slightly greater in cows of group A than group B. Interval to the onset of estrus after treatment was found non-significant (P>O.05) in both group cows. The length of estrus in group A was 21.54±1.65 hours whereas it was 19±0.88 hours in group
B. The difference in the length of estrus among the two groups was statistically non-significant (P>0.05). The conception rate of both groups was found to be 54.54% and 50% respectively, which was slightly higher than the second group. The mucus characteristics of both the groups were noted and 81.81% and 70% cows were found having typical fern like pattern and discharged clear (Transparent) mucus. No one cow having atypical fern pattern and cloudy discharge of mucus conceived. The behavioural signs and the physical changes were recorded. There was little difference in behavioural symptoms and physical changes among the groups. In group B the behavioural symptoms and physical changes were slightly more pronounced as compared to group A. The cost per animal for group A and B was 303 rupees and 276.50 rupees, economically group B technique seems to be profitable to the farmers, and by adopting this technique one can save money.
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7.
A Comparative Study On Reproductive Performance Of Friesian Cows, Due To Variable Environmental Influences,
by Rafique Tareen, M | Dr. Imtiaz Hussain Khan | Prof. Dr. Rashid Ahmed Chaudhary | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1996Dissertation note: The breeding records of 200 Friesian cows collected during 1977 to 1995 at Govt. Dairy Farm Quetta and 1983 to 1995 at Pishin, Balochistan were analysed. The parameters of reproductive performance like age at puberty, age at first calving, conception rate, service period, gestation period, calving interval, dry period and reproductive efficiency were determined. An effort was made to study the influence of the environmental factors on the reproductive traits.
The overall average age at puberty for 200 cows (150 heifer at Quetta and 50 heifers at Pishin) was 684.21±11.91 days at Quetta and 662.51±3.90 days at Pishin. Season of birth had highly significant effect on the maturity age. Winter born heifers matured earlier as compared to those born in other seasons. The data indicated that cows at Pishin matured earlier than that heifers at Quetta.
The age at first calving averaged 978.01 ± 17.39 days at Quetta and 958.90±16.311 clays at Pishin with range from 501-1100 days.
The overall average conception rate was 48.93% at Quetta and 50.33% at Pishin. The service period of cows at Pishin averaged 88.90±4.30 days and at Quetta, 81.30±3.3 days. Minimum service period was observed after Winter and maximum after Summer calving at Quetta. At Pishin minimum service period after Spring and maximum service period after Winter. The overall average length of calving interval was 464.20±4.9 days at Quetta and 361.11±38.00 days at Pishin.
Highly significant (P <0.05) difference in calving interval were recorded between Farms. Longest calving interval 374.50 days after Summer to Autumn calving and shorter calving interval 351.90 days after Spring calving were observed at Govt. dairy farm Quetta, but at Pishin longest calving interval 365.00 days after Autumn calving and shorter calving interval 356.10 were found days after Spring calving respectively. The dry period of Holstein Friesian averaged 69.7 ±2.9 days at Quetta and 65 ±3.50 days at Pishin. The result was highly significantly due to Farm difference. The overall reproductive efficiency of Holstein Friesian cows maintained at Govt. Dairy Farm, Quetta and Pishin, was 81±1.00% and 83±1.25%, respectively.
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8.
Comparative Efficacy Of Cloprostenol, Estradial And Gentamycine For The Treatment Of Endometritis In Buffaloes
by Chaudhry, Shafiq, M | Prof. Dr. Rashid Ahmad Chaudhry | Prof. Dr. Muhammad Aleem Bhatti | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1996Dissertation note: This study was designed to compare the efficacy of Cloprostenol, EstradioJ and Gentamycine for the treatment of endometritis in buffaloes.
Eighty buffaloes suffering from endometritis were selected amongst the animals brought for A.J. on repeat breeding at different A.I. Center/Sub-Centres in Lahore and Gujranwala city. After detailed gyanaecological examination animals were randomly divided into four treatment groups. Twenty buffaloes in group A were injected 500 ig of Cloprostenol (Estrumate). On day 8 of the oestrus cycle and cured animals were inseminated at the following oestrus. Twenty buffaloes in group B were given 3 intrauterine infusion of stilbestrol. On day 3, 5 and 7 of the oestrus cycle and cured animals were inseminated at the following oestrus. Twenty buffaloes in group C were given single dose of gentamycine (intrauterine infusion) on the day of the oestrus cycle and cured animals were inseminated at the following oestrus. Twenty buffaloes in group D served as control and were inseminated without giving any treatment. After two months all the animals were rectally palpated for pregnancy diagnosis. Seventy animals were tested for pregnancy diagnosis. While others were later transferred to untraceable places. The percent curative and conception rate observed in Cloprostenol treated group 'A' were 65% and 58.33% respectively similarly in group B Stilboestrol treated animals the curative rate was 75% and conception rate was 64.28%. In group 'C', the animals treated with Gentamycine, the curative and conception rate were observed 45% and 42.85%. In control group 'D' only 35.71% animals were conceived after insemination.
On the basis of curative and conception rates Stilboestrol was found better than Cloprostenol and Gentamycine for the treatment of endometritis buffaloes. The cost of treatment with stilboestrol is lower compared with Cloprostenol and Gentamycine. On the other hand a certain degree of invonvience remains with Stilboestrol therapy as intrauterine infusion demands special skill.
It is concluded that Stilbestrol and Cloprostenol results were signficanly higher as compared to treated with Gentamycine and control group.
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9.
Progesterone And Lh Profile Of Nili Ravi Buffaloes In Low Breeding Season
by Rizwan Nasr Cheema | Prof. Dr. Muhammad Aleem Bhatti | Prof. Dr | Prof. Dr. Ijaz Ahmed | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1996Dissertation note: In this project the main objective was to determine the oestrus activity of buffaloes during low breeding season. Out of six buffaloes 3 showed oestrus and out of these 2 repeated oestrus cycle. Both the primary and secondary symptoms of oestrus were clearly visible at oestrus. Length of the oestrus cycle was observed as 21.5 days with the average oestrus period 30.8 hours. The mean progesterone concentration in cyclic buffaloes on the day of the oestrus cycle (d=0)was 0.09 ng/ml and the highest serum progesterone concentration were observed on 16th day of oestrus cycle. In the non cyclic buffaloes the progesterone concentration remained around 1 ng/ml throughout the study. At the onset and the end of the oestrus the concentration of luteiningzing hormone was 3.9 ng/ml. Whereas at the beginning of standing heat average luteinizing hormone (LH) concentration 3.8 ng/ml. The surge of luteinizing hormone (LH) was 14.4 ng/ml observed. The average time of LH surge was 17.6 hours after the onset of the oestrus. End of oestrus time of following LH surge was 17.2 hours. Availability: Items available for loan: UVAS Library [Call number: 0512,T] (1).
10.
Induction Of Parturition In Buffaloes Using Dexamethasone In Conjunction With Prostglandin (Pgf2 Alpha)
by Arif Majeed | Dr. Imtiaz Hussain Khan | Prof. Dr. Muhammad Aleem Bhatti | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1997Dissertation note: The present study was intended to study the effect of prostaglandin (PGF2 alpha) and dexarnethasone on reproduction and production of Nili-Ravi buffaloes after parturition. For this purpose eighteen pregnant Nili-Ravi buffaloes were selected on the basis of their breeding history, with good health status, their lactation phases were 3rd, 4th, 6th, 8th, 9th and 10th respectively. These animals were divided into three equal groups viz group A, B and C, each comprising 6 animals. These animals were kept under uniform managemental and feedings conditions. Each buffalo in groups A and B were injected with 20mg dexamethasone and 526ig cloprostenol sodium (PGF2 alpha) intramuscularly on day 10 and 15 respectively before their expected term, while the buffaloes, in group C served as a control.
From the present study it was concluded that parturition can be induced at day 10 and 15 prior to their expected term successfully did not affect the production and reproductive efficiency of Nili-Ravi buffaloes. The use of PGF2 alpha and dexamethasone before parturition in buffaloes did not affect the milk yield of each buffaloes as compared with control group, and also the calve birth weight compared with control group. The only complication we found was the retention of foetal membrane, in all the treated buffaloes. No retention of foetal membrane was found in the control group. So the calving interval could be reduced by reducing the duration of pregnancy. It was also concluded that by induction the lactation period could be increased.
Availability: Items available for loan: UVAS Library [Call number: 0546,T] (1).
11.
Morphological Abnormalities In The Spermatozoa Of Cross Bred Cow Bulls During Summer Season
by Afzal, M | Prof. Dr. Muhammad Aleem Bhatti | Dr. Imriaz Hussain Khan | Prof. Dr | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1998Dissertation note: In this study twelve normally producing breeding bulls three of each Sahiwal and Friesian breeds and six bulls of 50% Cross-bred were selected. The mean volume of ejaculates were 4.87, 4.22 and 5.95m1 in Sahiwal, Friesian and Cross-bred, respectively. There is no significant difference among the volume of breeds during the months May, June and July. The semen concentration were 637.369, 606.554 and 312.806 million per ml in Sahiwal, Friesian and Cross-bred. However, the concentration of spermatozoa in Cross-bred were significantly lower (P<0.01) as compared to Sahiwal and Friesian bulls.
The overall average values for head abnormalities percentage were significantly lower (P <0.01) in Sahiwal vs Friesian and Crossbred (2.65 vs 7.20 and 8.90%) respectively during summer season.
The neck abnormalities percentage were significantly lower (P<0.01) in Sahiwal vs Friesian and Cross-bred 1.13 vs 3.52 and 5.59% respectively during summer season. Similarly the values of mid piece abnormalities percentage were significant higher in Cross bred vs Sahiwal and Friesian (2.04 vs 0.60 and 1.83%) respectively during summer season. The same results pertaining to protoplasmic droplets and tail abnormalities were obtained.
The overall average abnormalities percentage in spermatozoa of Sahiwal breed were significantly lower vs Friesian and Cross-bred (8.64 vs 21.32 and 27.67%) respectively during summer season. These results indicated that Exotic breed (Friesian) as well as Cross-bred have poor adaptability against the stress of the high temperature during summer season, so they produce lower quality semen.
Availability: Items available for loan: UVAS Library [Call number: 0549,T] (1).
12.
Role Of Single Injection Of Prostaglandin F2 Alpha On Breeding Efficiency Of Buffaloes
by Sajid Iqbal | Prof. Dr. Muhammad Aleem Bhatti | Dr.Imtiaz Hussain Khan | Dr.Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
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; Nature of contents: ; Literary form: Publisher: 1998Dissertation note: In the present study, a total of twenty Nili-Ravi buffaloes were divided into two equal groups. In group A ten buffaloes were administered with prostagladin F2 alpha (Lutalyse, Upjohn), 2 hours after calving. In group B, ten buffaloes were not given any treatment and designated as control. The reproductive organs of each experimental buffalo were rectally palpated on day 14 and day 21 postpartum. After that twice a week rectal palpation was carried out until the first postpartum oestrus.
The results of present study revealed that cervical and uterine involution was completed significantly (P < 0.05) earlier in group A as compared to group B (28.90± 1.79 and 35.40±3.95 days). There was no significant difference in the diameter of cervix, gravid and nongravid uterine horn at day 14 postpartum. A significant difference between the groups was obtained on days 21, 25 and 28 postpartum in the diameter of cervix and gravid horn. The corpus luteum (CL) of pregnancy regressed very rapidly following calving. The overall period required for complete regression of corpus luteum of pregnancy was (19.20±4.87 days) in treated group and (18.40±6.07 days) in control groups. The difference was significant.
Follicular activity resumed independently of uterine involution. It was, however, delayed slightly by the retained corpus luteum of pregnancy. The mean postpartum interval of initial follicular development was 21.20±5.71 days in treated and 28.20±8.75 days in control groups, respectively. The difference was statistically significant (P <0.05).
Postpartum oestrus interval was shortened in treated group (79.50±19.83 days) as compared to control group (103.0± 17.45 days) and the difference was significant (P<0.05). So it seems beneficial to administer prostaglandin F2 alpha in postpartum buffaloes to reduce the period for uterine involution and enhance the subsequent reproductive performance.
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13.
Use Of Prostaglandin (Pgf2 Alpha) To Induce Oestrus In Postpartum Non Cyclic Sahiwal Cows
by Amjad, M | Prof. Dr. Muhammad Aleem Bhatti | Dr.Imtiaz Hussain | Dr.Kamran | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 1998Dissertation note: The incidence of ovarian cysts in dairy cows in variously reported as ranging from 10-12 percent. Various factors are reported to influence the incidence of ovarian cysts. Factors that have been suggested are age, level of. milk production, nutrition, heredity, length of postpartum interval, frequency of examination of the reproductive tract and stress around the time of parturition. Ovarian cysts are a significant cause of reproductive failure because of prolongation of the interval from parturition to the first oestrus. Twenty four postpartum anoestrus Sahiwal cows at 85 days postpartum were selected and randomly divided into the groups viz group A and B. Group A (n=12) cows were injected 2m1 (5mg) prostaglandin F2 alpha (Prostavet, Virbac, France) intramuscularly (i/rn) at day 85 postpartum without palpation. A second injection of prostaglandin F2 alpha was given 11 days apart to those cows who had not responded to first injection. Rectal palpation was performed at 105 days postpartum to confirm the presence of corpus luteum on the ovary. A third injection was given at 118 days postpartum to those cows that had not responded to 1st and 2nd injection of PGF2c. The cows in group B were not given any treatment and served as control. The cows were artificially inseminated on the basis of standing heat 12 hours after the detection of heat. Oestrus detection was made by teasing, behavioural symptoms, physical changes and rectal palpation. In group A and B the oestrus response was 66.66% and 25% respectively. This response was greater in cows of group A than group B. There was difference in behavioural symptoms and physical changes among the groups. In group A the behavioural symptoms and physical changes were slightly more pronounced as compared to group B. Interval to onset of oestrus in group A was 96±15.08 hours whereas it was 25.0±1.0 days in group B. The length of oestrus in group A was 23.25±1.99 hours whereas it was 20.33± 1.45 hours in group B. The difference in the length of oestrus among the two groups was statistically non-significant (P> 0.05).
Group A animals exhibited oestrus during 89±0.62 days of postpartum. In group B animal exhibited oestrus at 110±0.19 days of postpartum and the difference was ignificant (P < 0.05). From the present study it is concluded that PGF2 treatment at 85 days postpartum leutolysed the luteal cyst and brings the cows in oestrus.
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14.
Ovulation Induction And Synchronization In Nili-Ravi Buffaloes With Prolonged Post-Partum Period
by Mohammad Atif ikram | Prof. Dr. Muhammad Aleem Bhatti | Prof. Dr. Rashid Ahmad Chaudhry | Faculty of Veterinary Sciences.
Material type: ; Format:
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Publisher: 2002Dissertation note: Estrus detection and correct tine insemination have been considered one of the important factors affecting the calving interval in 1)11 flab population. This study was undertaken to induce and synchronize ovu1a(on rather than esirtis in buffaloes. Ovsynch program ( ovulation synclronization) enables all fertile buffaloes to be bred at a fixed time by A.l.
Sixteen buffaloes of Nili-Ravi breed with a mean postpartum interval of 250±31.6 days were randomly divided into 2 equal groups viz A and B (ii=8). On day zero, Dalmeraline (25mcg/ml lecirilin; a GnRH analogue) was administered intramuscularly at the dose rate of 3cc. On day 7, 2cc Dalmazine (0.075mg/mi Cloprostenol) was injected intramuscularly. One day 9, second injection of Daimeraline (Lecirilin) was given intramuscularly at the dose rate of 3cc. Insemination was carried out 1 6 hours after second injection of GnRH analogue, Dalmeraline.
Buffaloes of group B were kept as control group. They were observed for naturally occurring estrus from day 0 to 26. Estrus was detected with the help of teaser bull. Artificial insemination was carried Out primarily on standing heat basis.
Buffaloes of group A responded to hormonal therapy and five out of 8 animals (62.5%) showed estrus behaviour and signs. All animals of group A were inseminated twice, once early in the morning and then in the evening with an interval of 12 hours.
Three animals (37.5%) from group A were conceived at induced and synchronized ovulation at first service, while one animal (12.5%) was conceived from group B.
It is concluded that the Ovsynch protocol is a useful regime in inducing and synchronizing ovulation with reasonably good conception rates. It is however, found that ovsynch protocol is also effective enough in buflaloes with longer postpartum intervals.
Availability: Items available for loan: UVAS Library [Call number: 0783,T] (1).
15.
Effect Of Collection Frequency On The Semen Quality Of Broiler Breeder
by Amjad Riaz | Prof. Dr. Muhammad Aleem Bhatti | Dr. Talat | Mr. Muhammad Amir Saeed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2002Dissertation note: In future, artificial insemination (AI) in poultry industry has a considerable importance because of the excellent results in fertility and hatchability. Increasing male utilisation in artificial insemination depends upon the optimum use of bids by increasing semen collection. The project was designed to scrutinise the effects of collection interval, testes weight and body weight on semen yield.
A total of ten broiler breeder males (35 weeks of age) were randomly selected. After providing ten-day sexual rest these were trained for semen collection by abdominal message technique. In study I the effects of various frequencies of semen collection on semen characteristics were investigated. All birds being collected at 48hours, 24 hours and 12 hours intervals. In each interval five consecutive collections were examined for motility percentage, semen volume and sperm concentration. Out of ten one cock gave no semen through out the study and 2 birds did not yielded semen at 12 hours interval and were excluded from the study.
No significant difference in collection intervals was established for percentage motility (P> 0.05), but semen collected at 12 hours interval was significantly lower than 24 hours interval in respect of semen volume and lower than 48 hours interval in respect of sperm concentration. Thus at 48 hours and 24 hours intervals we get significantly higher values of number of sperms per collection, number of motile sperms per collection and number of doses per collection than the values collected at 12 hours interval (P < 0.05). But difference for theses values at 48 and 24 hours collections intervals was non significant (P > 0.05).
The total number of semen doses over a six-day period increased linearly and significantly as the frequency of collection increased from once every two day to twice daily. Daily semen collection was found to be more suitable for Al programme at broiler breeder farm.
In study II daily semen output, paired testes weight and body weight were correlated. The semen output was found to be significantly correlated with testis weight (r =0.82) and body weight (r = 0.79), but no significant correlation was found between testis weight and body weight (r = 0.56).
In biometrical examination the weight of left testis was found significantly heavier than right testis but in respect of testicular volume no significant difference was observed between the two sides.
Availability: Items available for loan: UVAS Library [Call number: 0785,T] (1).
16.
Effect Of Various Extenders On Semen Characteristics Of Beetal Buck (Capra Hircus)
by Aoun Raza | Prof. Dr. Muhammad Aleem Bhatti | Dr.Muhammad | Dr.Muhammad Ashfaq Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2003Dissertation note: The artificial use of germ cells of genetcally superior bucks can enhance
the growth potential of goat population to meet the shortage of mutton meat in Pakistan for daily slaughtering, sacrificial events, skin, hair and goat milk too.
Beneficial use of superior germ cells can be made only when there is extension of life of germ cells for long periods, maintenance of motility of spermatozoa and increase in number of doses of ejaculate, for which an extender of choice has to be developed. Semen characteristics of forty ejaculates of bucks were evaluated. Pooled samples of ejaculates having motility estimates of at least 60% were used for evaluation. After washing of seminal plasma with physiological normal saline (20% ringer solution) and centrifugation at l000xg for 10 minutes to remove the sperm toxic factor Lecithinase-A. Pooled semen samples were extended in Tris yolk fructose citric acid (TYFCA), milk yolk (MY) and egg yolk citrate (EYC) extenders. Samples were extended using one
step extension at a ratio of 1:60 in such a way that each milliliter of semen contained 30x106 progressively motile spermatozoa. Finally extended semen samples were placed at 5°C and 37°C for evaluation of motility percentage after every 24 hours interval and livability (hours) and absolute index of livability o spermatozoa, respectively. Mean±S.E. values of ejaculates of bucks for volume, pH, mass motility, individual motility percentage, sperm cell concentration, live and dead percentage, sperm abnormalities was recorded and post-extension motility percentage at 5oC, livability (hours) and absolute index of livability of of' spermatozoa of pooled semen at 37°C was recorded. Significant differences were observed (P<0.01) in post extension motility percentage at 5°C, at all intervals except at 120 hours interval where deference vas non-significant (P>0.05) between milk yolk and egg yolk citrate extenders. Post extension motility percentage at 5°C was highest in TYFCA than MY and EYC e\tenders. Post extension livability (hours) at 37°C was significantly different among three extenders at a level of probability (P<0.05) but non-significantly different among three extenders at a level of' probability (P>0.01). Absolute index of' livability at 37°C shows significant differences (P<0.01) for all extenders under statistical analysis. Livability (hours) and absolute index of' livability was higher in TYFCA than MY and EYC extenders. Based on these results and effect of extenders on semen characteristics of Beetal Buck, Tris- Yolk-Fructose-Citric Acid was developed as an extender of choice for short term preservation of' semen of' Beetal Buck.
Availability: Items available for loan: UVAS Library [Call number: 0798,T] (1).
17.
Use Of Gonadotrophin Releasing Hormone For Estrus Synchronization In Nili Ravi Buffalo
by Abdul Majeed | Prof. Dr. Muhammad Aleem Bhatti | Prof. Dr. Rashid Ahmad Chaudhry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2001Dissertation note: Sixteen Nih Ravi buffaloes having postpartum period of 197±27.48 (Mean ±SE) days were randomly divided into 2 equal groups viz A and B (n=8). Estrus was induced and synchronized in buffaloes of group A by administering 75 mcg GnRH analogue (25 mcg/ml) intramuscularly on day 0 (first day of experiment). The buffaloes, showing estrus during day 0 to 7 were inseminated and were not given PGF2 alpha injection. The animals those did not exhibit signs of estrus till day 7, were injected with 0.150 mg Cloprostenol (0.075 mg/mi) intramuscularly. Buffaloes of group B served as control group and were not given any hormonal treatment. Animals in group A were observed for estrus from day 0 to 16 of the experiment, whereas the buffaloes of group B were bserved for natural estrus from day 0 to 26. Estrus was detected with the help of teaser bull. Artificial insemination was carried out mainly on the basis of standing heat. In group A, 4 buffaloes responded to the treatment and exhibited the estrus. One buffalo exhibited the estrus on day 5 following the injection of GnRH whereas three buffaloes exhibited the estrus on day 2, 4 and day 5 following the injection of PGF2 alpha. In group B, 2 buffaloes exhibited natural estrus, one on day 3 and the second on day 11 of the experiment.
Variation in the interval to estrus may be because of huge difference in the postpartum days of animals. The duration of estrus in group A (24.62±O.96h) was significantly different (P>O.05) as compared to group B (20.25±O.33h). No difference was observed between group A and B towards the expression of estrus symptoms. Two animals from group A, conceived at induced and synchronized estrus at first service, whereas one animal conceived from group B.
It is concluded that the select synch protocol is useful for synchronization of estrus in buffaloes with promising conception results. It is indicated that select synch protocol is also applicable for induction of estrus in buffaloes of variable postpartum intervals, it seems that select synch protocol will be an effective tool if used in cycling buffaloes during early postpartum days.
Availability: Items available for loan: UVAS Library [Call number: 0832,T] (1).
18.
Efficacy Of Gentamicin And Enrofloxacin In Endometritis In Postpartum Buffalo
by Muhammad Assad Javaid | Dr. Muhammad Amir Saeed | Dr. Muhammad Aleem | Prof. Dr | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2004Dissertation note: This study was carried out on 40 postpartum buffaloes suffering from endometritis with the objective that if gentamicin (@ 4mg/kg body weight, I/M) or enrofloxacin (@ 5mg/kg body weight, I/M) for 3 consecutive days is effective against the endometritis. Twenty postpartum, untreated, healthy buffaloes were kept as control (group C). The uterine characteristics and blood samples for haematological studies were examined before (day 0) and after treatment (day 4). Mucus samples were examined for fern pattern at first estrus before treatment and after treatment. Both the treatment did not differ significantly (P> 0.05) among them; however, they had significantly (P< 0.05) beneficial effect on cervical diameter, location of uterus, texture of uterine wall and symmetry of uterine horns. Intensity of uterine infection was cured sigtnificantly. The haematological values revealed a significant improvement (P<0.05 in EST, Hb and TLC in endometritic animals (group A and B) but the difference was non significant in control group. Whereas value of TEC was non significant in group A and C and significant improvement was observed in group B after treatment. Moreover, when we compare the 3 groups (A,B and C) to each other, non significant difference was observed among the Hb concentration in three groups. Whereas, ESr was not significant between group A,B and C, but was significant between groups B and C (P<0.05). in TEC, the values were significant difference in group A with C group B with group C. but no significant difference in group A with group B. and in TLC, there was significant difference in group A with group B and group ?A with group C, but no significant difference in group B with group C. similarly in DLC the values of eosinophils were significant in group A. but no significant difference in group B and group C in before and after treatment. In neutrophils, there were significant differences in values of group B but no significant difference in group A and group C. also in lymphocytes, significant difference was found in group B, but was not no significant difference in group A and C in before and after treatment. As in basophils, monocytes, there was no significant difference in before treatment and after treatment. Furthermore, when these DLC value were compare with each other after treatment, there was significant difference in neutrophils and lymphocytes within group A with B, A with C and B with C. in monocytes, significant difference was found in A with C group and group B with C, but no significant difference in A with B. In eosinophils, no significant difference was found in all three groups. And in basophils, significant difference was found in group A with C and B with C. but no significant difference in A group with B group. The physical signs (uterine tone, vulvular swelling, hyperemia, and wetness in vagina, switching of tail, mucus discharge) and behavioural signs (Micruration, rising of tail, bellowing, mounting and restlessness of estrus were studied. After treatment estrus was observed in 45% of group A and 55% of group B buffaloes. The pregnancy rate was 60% in group A and 50% in group B buffaloes. This was comparable to the pregnancy rate in group C buffaloes (62%). And there were no significant difference in the conception rates. It is concluded that both gentamicin and enrofloxacin are equally effective for the treatment of postpartum endometritic buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 0847,T] (1).
19.
Effect Of Norgestomet And Gnrh On Besumptio Nof Cyclicity And Pertility In Early Postpartum Non-Suckled Buffaloes
by Attiq Ullah | Dr. Muhammad Amir Saeed | Dr. Khalid | Prof. Dr. Nasim Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2004Dissertation note: Delayed resumption of postpartum estrus activity is the most vital factor responsible for poor reproductive efficiency of dairy buffaloes. This study was designed to induce ovulation in early postpartum buffaloes (30-37 days). Fifteen Nili-Ravi buffaloes with a mean postpartum period 32.6±2.72 were randomly divided into three equal groups A, B and C (n=5) on the basis of parity, postpartum period, age and milk production. The animals in group A were given Norgestomet implant at day 32.8±2.94 postpartum period for 9 days. One day after removal of implants animals were given Dalmeralin (Leciren). The animals of group B given only Norgestomet implant at day 32.4±2.50 postpartum for 9 days. Group C animals acted as control and did not receive any treatment.
Same number of animals (4/5) exhibited estrus in both groups A and B. None of the buffaloes in-group C showed estrus. It is concluded that treatment of norgestomet or norgestomet + GnRH in early postpartum buffaloes, are able to induce estrus, however, fertility remain compromised.
Availability: Items available for loan: UVAS Library [Call number: 0884,T] (1).
20.
Effect Of Sperm Storage Tubules Secretions From Pre-Layer Hen On Cockerel Sperm Clumping And Motility
by Iqbal Munir | Prof. Dr. Ijaz Ahmed | Dr. Amir Saeed | Prof. Dr. Zafar Iqbal Chaudhry | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2004Dissertation note: Artificial insemination (AT) in the poultry industry has considerable importance because of better results in fertility and hatchability. Increasing male utilization in artificial insemipation depends upon the optimum use of semen by suitable diluting media to increase the volume of ejaculate and to preserve fertility.
In the present study, the effect of sperm storage tubules secretions on percentage motility and extent of clumping of sperms was noticed. An optimum osmotic pressure 375 mOsm with pH 7.0 was used to preserve the cockerel's semen at 5°C. A total of 20 meat Breeder cockerels were randomly selected. After providing 10-days sexual rest, they were trained for semen collection by abdominal massage technique. Three birds failed to produce good quality semen. These birds were removed from the study.
Semen from seventeen Meat Breeder Cockerels was pooled. After
macroscopic evaluation, the pooled semen was divided into 4 groups.Group A was diluted with Modified Van Wambeke diluent with addition of sperm storage tubules secretions. Group B was diluted with the above diluent without SST secretions. Group C was diluted with saline solution with addition of SST secretions while group D was diluted with saline solution without SST secretions. These four groups were stabilized at 375 mOsm osmotic pressure in pH 7.0 and stored at 5°C. The diluted semen samples were examined for percentage motility and extent of clumping. After 72 hours of semen storage (5°C), group A showed significantly (P<0.05) higher motility as compared to groups B, C and D.
The extent of clumping was higher (P <0.05) in group D as compared to groups A, B and C. However, group A showed less (P <0.05) clumping upto 64 hours as compared to groups B, C and D.
The results of the present study suggested that at 375 mOsm, pH 7.0 the cockerels semen stored at 5°C diluted wiih Milk based extender and saline solution with addition to SST secretions proved to be suitable for short-term preservation of Meat Breeder Cockerel semen.
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21.
Effect Of Cold Shock On Frozen-Thawed Spermatozoa Of Buffalo & Cow Bulls
by Syed Amer Mahmood | Prof. Dr. Ijaz Ahmed | Dr.Azhar | Dr.Muhammed Amir Saeed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2005Dissertation note: Effect of cold-shock on frozen-thawed bovine spermatozoa was measured in terms of motility, viability, plasma membrane and acrosome . integrity. Single ejaculates each from seven Nili-Ravi buffalo bulls and 7 Sahiwal cow bulls were processed for freezing. Three straws of 0.5ml (for each parameter) from each bull were thawed and pooled at 3rC before and after cold-shock at 4°C for 2 min. Individual motility was observed by phase contrast microscope before and after cold-shock. Before and after cold-shock, sperm viability, plasma membrane and acrosome integrity were evaluated by supravital stain, hypo osmotic swelling test and normal acrosomal reaction, respectively. Two hundred sperm were counted for each parameter before and after cold-shock. Comparison of before and after cold shock values showed that cold shock had no effect (P>0.05) on motility (64.0±1.88 vs 60.4±2.56), viability (141.1±4.39 vs 124.5±8.03), plasma membrane integrity (83.51±5.82 vs 71.19±4.31) and acrosome integrity (148.3±2.36 vs 141.1 ±2.85) of buffalo bull spermatozoa. Comparison of before and after cold shock values of cow bull spermatozoa indicated that cold shock had significant (P<0.05) effect on motility (59.2±1.05 vs 41.9±.12), viability (140: 14±2.94 vs 90.5±2.73), and plasma membrane integrity (62.3±4.28 vs 47.24±3.71) but no effect on acrosome integrity (147.9±2.21 vs 140.6±2.40). In conclusion, cold shock had significant detrimental effect on cattle bull spermatozoa but no effect on buffalo bull spermatozoa.
Availability: Items available for loan: UVAS Library [Call number: 0910,T] (1).
22.
Effect Of Osmotic Pressure On The Membrane Integrity Of Frozen-Thawed Buffalo Bull Spermatozoa
by Muhammed Irfan-Rehman Khan | Prof. Dr. Ijaz Ahmed | Dr.Azhar | Dr.Muhammed Mushtaq | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2005Dissertation note: In the first experiment, semen from seven Nili-Ravi buffalo bulls was used to study plasma membrane integrity of freshly collected (raw) and frozen-thawed sperm using the hypo-osmotic swelling test (HOST). For this purpose, percentage motility, integrity of plasma membrane and acrosome was assessed by a phase contrast microscope, HOST plus eosin-nigrosin staining and normal apical ridge test. respectively. 50fJI each of raw and frozen-thawed semen was mixed with 500fJI of 50, 100, 150, 190 or 250 mOsm hypo-osmotic treatments of sodium citrate plus fructose and incubated at 37°C for 1 h. Integrity of sperm plasma membrane was assessed before and after hypo-osmotic treatments to estimate the extent of damage for each hypo-osmotic treatment. In raw semen, the number of swollen sperm was higher (P<0.05) at 50, 100, 150 and 190 mOsm as compared to 250 mOsm. A positive but non-significant correlation (P>0.05) was found between percentage of swollen and live sperm at 100, 150, 190 mOsm. Similarly, a positive but non-significant correlation (P>0.05) was found between percentage of swollen sperm and motility at 50, 100, 150, 190 and 250 mOsm. In frozen-thawed semen, the number of swollen sperm w.as higher (P<0.05) at 50 and 100 mOsm as compared to 150, 190 and 250 mOsm and this number decreased significantly (P<0.05) and gradually from 82.6±5.99 at 150 mOsm to 69.7±5.49 at 190 mOsm and 42.6±4.07 at 250 mOsm. A positive but non-significant correlation (P>0.05) was found between percentage of swollen and live sperm and between percentage of swollen sperm and motility at 50, 100 150, 190 and 250 mOsm. The number of sperm with intact acrosome did not differ (P>0.05) in raw and frozen-thawed semen among treatments. Percentag~ motility in raw semen was highe~' (81%) as compared to frozen-thawed semen (60%). Damage to plasma membrane'was higher (P<0.05) at 50 mOsm (59% raw vs. 70% frozen-thawed) as, compared to other hypo-osmotic treatments, while minimum damage occurred at 250 mOsm (4.1% raw vs. 9.7% frozen-thawed). In the second experiment, hypoosmotic swelling test (HOST) plus eosin-,nigrosin staining and normal apical ridge test (NAR) were used to determine integrity of plasma membrane and acrosome of raw, diluted (cooled to 5°C) and frozen-thawed sperm. Semen from seven bulls was used in this study. For diluted and frozen-thawed sperm, three straws were pooled at 37°C. Percentage motility of raw, diluted and frozen-thawed sperm was assessed using a phase contrast microscope. 501-11 each of raw, diluted and frozen-thawed sperm was mixed with 5001-11 of 50, 100, 150, 150, 190 or 250 mOsm hypo-osmotic treatments of sodium citrate plus fructose and incubated at 37°C for 1 h. Total number of intact (live) sperm of raw, diluted and frozen-thawed semen was assessed before HOST. Diluted sperm showed higher (P<0.05) swellings of plasma membrane at 50
and 100 mOsm than raw sperm. Similarly, swellings of diluted sperm were' higher (P<0.05) than frozen-thawed sperm. Swellings of raw sperm were significantly higher (P<0.05) at 100, 150, 190 and 250 mOsm than frozenthawed sperm. A significant decrease (P<0.05) was found among percentage motility of raw (81±1.57), diluted (69.6±2.24) and frozen thawed (60.1 ±1.34) sperm. Live sperm were higher (P<0.05) in raw (174.4±7.33) and diluted semen (175.6±3.76) as compared to frozen-thawed semen (142.3±4.84). Although integrity of the acrosome of raw, diluted and frozen-thawed sperm did not differ (P>0.05), significan~ variation was found within bulls. In conclusion, fresh and frozen-thawed sperm behaved differently to HOST and the number of swollen sperm was higher in raw as compared to frozenthawed semen. Plasma membrane' integrity of raw and diluted sperm was compromised during freezing and thawing. However, freezing had no effect on acrosome integrity. Moreover, 100, 150 and 190. mOsm were found suitable to perform HOST.
Availability: Items available for loan: UVAS Library [Call number: 0911,T] (1).
23.
Effect Of Hormone Addition (Pgf 2 Alpha) And Oxytocin In Semen Extender On Post Thaw Semen Quality And Fertility in Nili Ravi Buffaloes
by Dr. Rafaqat Ali | Dr. Muhammad Amir Saeed | Dr. Asim Aslam | Prof. Dr. Nasim Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2005Dissertation note: This project was executed to improve the semen quality during freezing process and to improve subsequent fertility rate in Nili-Ravi buffaloes with hormones (PGF2a and oxytocin) supplementation. Pooled semen from Nih Ravi buffalo bulls (n2) was divided into 8 equal parts after complete evaluation and subjected to the hormonal treatments. PGF2a (Lutalyse®) @ 2.5mg, 5.0mg and 7.5mg and oxytocin (Cintocinon®) @ 2.5 I.U, 5.0 I.U and 7.5 I.U/lOOml of diluted semen were added. One group (-ye control) received indomethacin @ 20mg + PGF2a @ 5.Omg/lOOmi of diluted semen. One group remained without any treatment (+ve control). Semen was cooled, filled in 0.5ml straws, equilibrated at 4°C for 4 h ours and frozen i n liquid nitrogen. After 24 hours of deep freezing, semen was thawed and evaluated for percentage motility of spermatozoa, plasma membrane integrity (HOS assay), acrosome integrity (NAR), viability (Live/Dead), longevity (hours) and fertility. Four (4) straws from each treatment group were thawed and pooled in 5m1 cuvette in water bath at 37°C and evaluated for quality parameters. Twenty five (25) straws from each treatment group were used to inseminate the buffaloes in standing estrus at 3 A.I centers (Phool nagar, Changa manga and Kot radlia kishen) in district Kasur. Pregnancy was checked 60 days post insemination. Data collected was presented as mean ± SEM, treatment groups were compared using ANOVA, unpaired two sample test and Pearson correlation at 5% level of confidence interval using Minitab® computer software. Results o f this study revealed that addition of both Lutalyse® (PGF2a) and Cintocinon® (oxytocin) did not show significant (P>0.05) improvement in any quality parameters measured and a non significant correlation was observed between treated groups and control except indomethacin and viability of spermatozoa where a significant negative correlati9n (r = -0.980) was found. However, blocking of seminal prostaglandins with indomethacin shows significant (P<0.05) deterioration in percentage motility, plasma membrane integrity, viability and longevity of spermatozoa but acrosome integrity remain unchanged. Results of fertility trial showed significant difference (P<0.5) among treatment groups. In conclusion, we can say that although hormonal addition did not improve semen quality but improve fertility rate, therefore, the importance of prostaglandins in semen can not be neglected. The W nature and physiological amount in buffalo semen should be investigated and maintained by exogenous addition after dilution during processing to maintain post thaw semen quality and fertility. However, it is suggested that pure forms of hormones should be used to add in semen instead of Lutalyse® as it contains 1.56% alcohol which is supposed to be detrimental to spermatozoa.
Availability: Items available for loan: UVAS Library [Call number: 0923,T] (1).
24.
Molecular Detection Of Programmed Cell Death (Apoptosis In Fresh And Cryopreserved Buffalo Sper Matozoa)
by Daulat Raheem Khan | Prof. Dr. Nasim Ahmad | Dr. Irshad | Dr. Muhammad Amir Saeed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2006Dissertation note: The objectives of present study were a) to validate annexin V/Pl assay, for buffalo sperm, using graded doses of camptothecin through fluorescent microscopy (Exp. 1) and b) to determine the effect of stages of cryopreservation on apoptosis (PS externalization, using annexin V/PT assay), motility and plasma membrane integrity in buffalo sperm (Exp.2). In the first experiment graded doses of camptothecin were used (n=2) in different aliquots of sperm suspension (1x106/mL) for induction of apoptosis. Higher dose levels of camptothecin (10.0 iM and 20 jiM) resulted in inducing apoptosis (P<O.05) compared to the lower (5tM) dose or control. In the second experiment semen samples (n=9, from three bulls) were cryopreserved using vapor freezing. Annexin V/PI assay for apoptosis, visual assessment for percentage motility and hypo-osmotic swelling test for plasma membrane integrity were employed at various stages of cryopreservation (i.e. fresh, after equilibration and post thaw). The mean percentage of apoptotic, necrotic and viable sperm did not differ between fresh and after equilibration stages. However, freezing and thawing increased (P<0.05) the percentage of apoptotic sperm (25.4±0.6 vs. 36.5±1 .9) while decreased (P<0.05) the necrotic (29.7±0.7 vs. 35.1±1.2) and viable sperm (37.2±1.3 vs. 32.8±1.9, (P<0.07). Similarly, freezing and thawing decreased (P<0.05) the mean percentage motility and plasma membrane integrity, compared to other stages. Based upon the difference in initial and post thaw values of two variables (percent motility and percent apoptosis) it is concluded that apoptosis contributes more than 50% sperm motility loss during the process of freezing and thawing in buffalo.
Availability: Items available for loan: UVAS Library [Call number: 0933,T] (1).
25.
Effect Of Supplementation Of Different Types Of Fat On Buffalo Semen Production And Quality
by Muhammad Adeel | Prof. Dr. Muhammad Aleem Bhatti | Dr. Makhdoom Abdul Jabbar | Dr.Amir Saeed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2007Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0969,T] (1).
26.
Assesment Of Buffalo Semen By Reduction Assay
by Muhammad Iqbal | Prof. Dr. Muhammad Aleem Bhatti | Prof.Dr.Ijaz Ahmad | Prof.Dr.Talat | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2008Dissertation note: In this study twenty Nili Ravi buffalo bulls were used. Semen was collected once weekly and each collection comprised two ejaculates .. Total volume of the semen was observed using graduated tube and the concentration by Spectrophotometer. Both the ejaculates were pooled and a representative sample of each collection was evaluated for viability using MTT Reduction Assay, Eosin and Nigrosin Staining, Hypo Osmotic Swelling Test and Motility.
The results of the study shows high correlation (r = 1.00) between the MTT reduction rate and the sperm viability. When the %age of MTT viable sperm cells were compared with the E&N, HOST and Motility a negative correlation was found suggesting the physiological difference between the tests. On the basis of this study it can be concluded that the MTT reduction Assay is an objective test and more reliable than the other tests which are more subjective and can be influenced by the experience of the indivi~
MTT Reduction Assay is more reliable test for the assessment of viability as it is simple and inexpensive. The results of our study suggest additional advantages of this test in evaluation ofNili Ravi Baffalo semen. Many samples and its replicates can be measured in the same time. It can be used successfully in routine analysis, where time, costs and practicability are important
Availability: Items available for loan: UVAS Library [Call number: 1014,T] (1).
27.
Comparison Of Reproductive Efficiency Of Beetal Goats In Different Management
by Muhammad Younos | Prof. Dr. Muhammad Aleem Bhatti | Prof.Dr.Hafiz | Prof.Dr.Ijaz Ahmad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2008Dissertation note: The present study was conducted at two fanns (1) NH goat fann at Raiwind and (2) BN at Okara. The study consist the reproductive efficiency of beetal goats in different management systems. In this study we compared two management systems of goat production i.e. (1) seasonal (2) year round breeding were compared on the basis of reproductive efficiency and growth parameters, moreover cost effectiveness of the both systems were compared on the basis of their outcomes.
In this study, reproductive and productive parameters of n = 72 adult goats + 2 bucks in each flock were collected and analyzed. Data regarding pubertal and twinning of their progeny born during a period of a year were collected and compared. Goats of same breed and comparable ages and body weight, body condition scoring were used in this study. Study was carried out at similar period of time for one year.
The conception rate was higher at NH than BN goat fann. It was 0.809± 0.058 in BN and 0.88 ± 0.066 in NH respectively. The birth weight of male and female kids ofBN fann was higher than NH kids. It was (3.824 ±0.083 kg) in BN kids (3.513 ± 0.20 kg) in NH kids respectively. While the weight of female kids were (3.503 ±0.083 kg) in BN kids and (3.456 ± 0.10 kg) in NH kids. The age at puberty was higher at BN (245.3 ± 2.9 days) than NH goat fann (242.4 ±1.9 days). The kidding interval at BN was higher than NH flock. It was (314 ± 20 day) and (224.8 ± 0.915 days) days respectively. The post partum anestrous exhibited only in NH does, because in this fann the buck was free all around the year and after (50.0± 3.95 days) the does mate if they are in estrous, while in BN fann, bucks were free during the season. The body condition scoring (1-5 point) was higher in NH goats (2.44 ± 0.14) than BN goat fann (1.91 ± 0.11). The Body weight of does between both flocks were higher in BN (42.47 ± 0.92) than in N H goat farm (41.10 ± 1.9). The high concentrate feeding intake between two farms showed that the concentrate feed intake was higher in BN 491.5 ± 8.5 gm per day per goat than N H goat farm (146 ± 30 gm per day.
CONCLUSION
It is concluded that the reproductive efficiency of Beetal goats in N H goat farm was higher than BN farm~ because the seasonal breeding at BN has limited the kidding frequency. Where as~ all year round breeding was efficient and the kidding distribution almost doubled in the same year. The cost of feeding was higher in BN than NH goat farm~ because they provide concentrate daily~ but the cost of labour and miscellaneous was higher in NH than BN goat farm. The management of feeding~ green fodder was higher and there were sufficient green fodder for whole year in NH goat farm. The reproductive performance of Beetal goats can be improved by good managemental systems and all year round breeding.
Research and development efforts can significantly improve reproductive efficiency of goats and simultaneously enhance the livelihood of the poor. Structuring the whole system via adapted reproductive techniques (thus allowing high levels of productivity). Findings of this study would be useful to determine the viability of systems on the basis of reproductive efficiency and may provide basic guidelines to establish goat farms in the future.
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28.
Use Of Antioxidate Containing Extender To Improve Post Thaw Quality Of Canine Semen
by Zia Ullah | Prof. Dr. Ijaz Ahmad | Prof. Dr. Muhammad Aleem.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2010Dissertation note: plasma membrane integrity, acrosomal integrity and viability of spermatozoa) using the Labrador retriever dog frozen semen.
Dog is a unique animal in terms of its semen ejaculation which is in fractions with a highly variable sperm concentration in different fractions as compared to other mammalian species like bull, boar, ram, human which have their ejaculate as a single fraction. In this study, BHT was found to improve post thaw motility, sperm viability, plasma membrane integrity and the acrosomal integrity at an inclusion level of 1.0mM in the extended semen. However, the higher concentrations than this were found to have detrimental effects on the parameters mentioned above. The reasons for these detrimental effects could be the change in osmolarity of the semen that varies in different species. The ROS production is associated with normal physiological functioning of the spermatozoa and when a semen sample is diluted the endogenous antioxidant mechanism of the semen is compromised and addition of exogenous antioxidant is needed to equilibrate. Therefore the addition of BHT at an inclusion level 1.0mM in study was found optimum and higher concentrations of this had detrimental effect on the spermatozoa, which could be due to two reasons as explained by Ijaz et al., 2009, i.e., firstly higher concentration of BHT reduce the physiological functions of the spermatozoa by extremely reducing the ROS which are needed to a certain level for physiological function of the spermatozoa and secondly it increases the fluidity of the plasma membrane above the desired levels thus making it prone to more damages.
In conclusion, BHT was found useful for dog spermatozoa at inclusion level 1.0mM, however, further studies are required to investigate the fertility of the canine semen having inclusion levels of BHT.
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29.
Use Of Butylated Hydroxytoluene (Bht) As Antioxidant In Buffalo Semen Extender
by Tayyab Munir | Prof. Dr. Muhammad Aleem | Prof. Dr | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The Buffalo is the major milk producing animal in Pakistan. Nili-Ravi breed of buffalo is the one of the best breeds of the world. Buffalo contributes seventy percent of total milk production in Pakistan. The excellent buffalo bulls are few in number and their semen volume and sperm concentration is also low as compared to cattle. The animal has lower fertility particularly when inseminated with frozen semen. There are many reasons for poor fertility of buffalos and one of the most important reasons may reasons for poor fertility of buffalos and one of the most important reasons may be the production of Reactive Oxygen Species (POS) from the plasma membrane of spermatozoa causing damage to the membrane integrity due to lipid peroxidation. The addition of optimum level of antioxidants like BHT to buffalo semen extender before freezing may be answer to this problem. The main objective of the present study was to determine the level of total of antioxidants in the semen and to optimize the quantity of BHT in the semen extender of the Nili Rave Buffalo bulls.
In this study semen from Nili-Ravi buffalo bulls ( n=10) was collected twice per week and diluted with Tris egg yolk citrate extender. Antioxidant BHT was added to the extended semen at the rate of 1.0mM, 1.0 mM and 2 mM while Control was containing no BHT. The semen was visually analyzed under phase contrast microscope for motility, vitality (Eosin/Nigrosin staining), plasma membrane (HOS assay) and acrosomal integrity (NAR), at three stages i.e. after dilution, before freezing and after freezing. For the determination of total antioxidant capacity (TAC), semen samples were transported under ice to Spectophotometric Laboratary, GC. University Lahore. Data collected was presented as mean= SEM. Treatment groups were compared using one way ANOVA and Results were compared by using Duncan Multiple Range Test using SPSS (version 16.0) ; SPSS Chicago IL.
Result of this study show significant (P< 0.05) improvement in semen motility, vitality, plasma membrane and acrosomal integrity at 1.5 mM BHT concentration as compared to Control, however, insignificant difference was noticed with in treated groups and with in bulls. Maximum TEAC value (2.42) was observed in the extended semen containing two mM BHT and minimum TEAC values (0.58mM) in the post thaw semen sample with BHT.
It is concluded that addition of antioxidants in the buffalo semen improve the semen quality before and after freezing. Fortification of buffalo semen extender with BHT (@ of 1-1.5 mM) improved post thaw semen quality and hoped to increase the fertility of buffalo. However, field fertility trials need to be carried out for confirmation.
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30.
Effect Of Two Synchronization Protocols (Ovsynch Va 2Pg)And Gnrh Pn Day Of Breeding On Reproductive Performancein
by Hasan Riaz | Prof. Dr. Nasim Ahmad | Dr. Mian Abdul Sattar | Prof. Dr.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The main objective of the present study is to compare the two synchronization protocols (Ovsynch vs 2PG) on estrus response, follicular development, ovulation, pregnancy rate and fecundity in Beetal and Teddy breeds of Pakistan. The secondary objective is to determine the efficacy of GnRH at the time of breeding on pregnancy rate in Beetal goats. Experiment 1 was conducted with a total of 28 goats during breeding season (September-October) at Livestock Production Research Institute, Bahadurnagar, Okara. The goats were randomly allocated in one of two treatment groups, Ovsynch and two PGF treatments. The Ovsynch treated does received an intramuscular injection of GnRH analogue 12.5µg lecirelin (Dalmarelin®, Fatro, Italy), on "Day 0". On "Day 7" does were treated with injection of PGF2? analogue 37.5µg d-cloprostenol (Dalmazin®, Fatro, Italy,) followed by a second injection of 12.5µg lecirelin (Dalmarelin®, Fatro, Italy), after a 48h period. Animals in the double PGF group received two injections of 37.5µg d-cloprostenol (Dalmazin®, Fatro, Italy), 10 days apart. In order to determine the estrous response and its duration, two aproned bucks were used twice daily for 30 minutes after the last PGF2? injection in both groups until the termination of estrous signs. All goats were naturally bred 12h after the onset of estrus. Follicular and luteal activity was monitored by transrectal Ultrasonography (ALOKA, SSD 900 Japan) with the aid of a 7.5-MHz linear array transducer daily- from the start of treatment, until estrus and then twice a day until the cessation of large follicles (apparent ovulation). On day 7 after breeding, all goats were re-examined by transrectal ultrasonography to determine the ovulation rate, based upon the number of CL's present. Pregnancy rate and fecundity (no of embryos per doe) was again determined at Day 30 after breeding by transrectal ultrasonography. Experiment 2 was performed on 25 goats. These does were randomly allocated to two groups (GnRH, n=11 and Control, n=14). GnRH does received 12.5µg lecirelin on the day of natural breeding (12h after standing estrus), while the control does did not receive any treatment. Timing of ovulation, pregnancy rate and fecundity were assessed by transrectal ultrasonography as in Experiment 1. Estrus (Experiment 1) was detected in 10 out of 14 does (71%) in Ovsynch group, while all 14 goats (100%) of double PGF responded group. The interval from the last PGF injection to estrus was 48.0 ± 2.6h in the Ovsynch group, while 36.0 ± 1.2h in the double PGF group (P<0.05). The interval from the last PGF injection to ovulation did not differ significantly between the Ovsynch (73.0 ± 4.8h) and double PGF (66.0 ± 2.7h) groups respectively. Similarly the interval from standing estrus to ovulation was not significant between the Ovsynch (24.0 ± 3.7h) and double PGF (30.0 ± 2.7h) treatments respectively. Estrous duration was similar in both treatment groups (44.7 ± 4.9h for Ovsynch and 47.1 ± 2.9h for the 2PGF). The size of ovulatory follicle was similar in both treatment groups (Ovsynch, 7.1 ± 0.2mm; 2PGF, 7.1 ± 0.1mm). On day 7 after breeding, the ovulation rate (no. of CL's on the ovary) were similar in both the Ovsynch (1.6 ± 0.2) and double PGF (2.0 ± 0.2) groups. The pregnancy rate as determined by transrectal ultrasonography on day 30 (Fig. 1) revealed 6/10 (60%) in the Ovsynch and 11/14 (78%) in the double PGF groups respectively (not significant). Fecundity was similar for both treatment groups (1.6 ± 0.2). In Experiment 2 the interval from standing estrus to ovulation recorded was not significant (31.2 ± 2.9h and 36.0 ± 5.3h), the ovulatory diameter (6.8 ± 0.1mm, 7.2 ± 0.5 mm (P<0.05), the pregnancy rate (54% (6/11) and 64% (9/14), and fecundity (1.5 ± 0.5, 1.7 ± 0.5) were not significant between GnRH treated and control does respectively (Table 2). From these experiments it can be concluded that the Ovsynch protocol, which is a non-steroidal and safe approach for estrous synchronization appears to be similar to the double PGF injection protocol, in terms of the reproductive performance in goats. However, this needs to be tested on larger number of animals. Furthermore, the use of GnRH at the time of breeding does not improve the reproductive performance in goats. The data generated has important implications for commercial goat farming in the developing countries.
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31.
Effect Of Cholesterol Addition To Buffalo Bull Semen During Cryopreservation Procedures On Post-Thaw Survival Of Spermatozoa
by Ahmad Yar Qamar | Dr. Mian Abdul Sattar | Prof. Dr | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Cholesterol is an important constituent in the membrane fluidity regulation. This role becomes
more important in the cryopreservation of sperm cells where destabilization of plasma membrane
leads to intracellular ice formation causing death of the cell. The objective of the present study
was to verify if addition of cholesterol in semen extender has a beneficial effect on post-thaw
semen quality in buffalo bulls. This study was carried out at Al-Haiwan Sires, Sahiwal, Pakistan.
Cholesterol was added to Tris-citric acid semen extender in the form of cholesterol-loaded
cyclodextrin (CLC). Semen was collected by using artificial vagina (420 C) from four buffalo
bulls. Split pooled ejaculate (n = 7), having more than 60 % visual sperm motility, were diluted
at 370 C in extender containing CLC either 3mg Iml (LOW), 4 mgl ml (MED), 5 mg Iml (HIGH)
or without (CON). Motility of semen samples before freezing was checked and cryopreservation
was done through routine procedures. Beneficial effect of CLC in cryopreserved semen was .
assessed by post-thaw motility (%), live spermatozoa (%), morphological abnormalities (%),
normal acrosornes (%) and plasma membrane integrity (%) in four groups. Analysis of variance
revealed that motility before freezing was significantly (P<0.05) more in MED (68.93 ± 0.51 %)
as compared to CON and HIGH but non-significantly higher than LOW. Percent post thaw
motility (PTM) from semen samples containing MED concentration of CLC was the highest
(61.43 ± 0.74 %; P < 0.05) followed by LOW (50.36 ± l.15 %; P < 0.05) or HIGH (38.57 ± 2.66
%; P < 0.05) (Table 1). Percent PTM was the lowest in CON (32.86 ± 2.07 %; P < 0.05) semen
samples. Mean plasma membrane integrity (PM I) from semen samples containing MED
concentration of CLC was the highest (59.36 ± 1.32 %; P < 0.05) followed by LOW (51.93 ±
1.32 %; P < 0.05) or HIGH (44.00 ± 1.65 %; P < 0.05) and was least in CON (41.14 ± 1.71 %; P
< 0.05) semen samples. Mean normal acrosomes from semen samples containing MED
concentration of CLC was the highest (52.93 ± 1.78 %; P < 0.05) followed by HIGH (40.57 ±
2.01 %; P < 0.05) and CON (34.93 ± 2.89 %; P < 0.05) semen samples. Buffalo bull semen in
MED & LOW had significantly (P < 0.05) higher live sperm percentage (69.64 ± 1.84 & 61.43 ±
1.62, respectively) as compared to HIGH and CON samples. CON samples had significantly
(P<0.05) higher morphological abnormalities (9.86 ± 0.14 %) as compared to LOW & MED but
non-significantly higher as compared to HIGH samples. All the variables under study had strong
positive correlation (P < 0.01) with each other except morphological abnormalities having strong
negative correlation (P < 0.01) with all others. It is concluded that addition of cholesterol in
MED concentration (4 mg CLCI ml of semen extender) to buffalo bull semen can improve post-
thaw semen quality.
Conception rate of AI in buffaloes is generally lowered than in cows. Cholesterol is an
important constituent in the membrane fluidity regulation. This role becomes more important in
the cryopreservation of sperm cells where destabilization of plasma membrane leads to
intracellular ice formation causing death of the cell. The objective of the present study was to
verify if addition of cholesterol in semen extender has a beneficial effect on semen post-thaw
quality in buffalo bulls. This study was carried out at Al-Haiwan Sires, Sahiwal, Pakistan.
Cholesterol was added to Tris-citric acid semen extender in the form of cholesterol-loaded
cyclodextrin (CLC). Semen was collected by using artificial vagina (42° C) from four buffalo
bulls. Split pooled ejaculate (n = 7), having more than 60 % visual sperm motility, were diluted
at 37° C in extender containing CLC either 3mg Iml (LOW), 4 mgl ml (MED), 5 mg Iml (HIGH)
or without (CON). Motility of semen samples before freezing was checked and cryopreservation
was done through routine procedures. Further examination was done to check post-thaw motility
(%), live spermatozoa (%), morphological abnormalities (%), normal acrosomes (%) and plasma
membrane integrity (%) using phase contrast microscope and hypo osmotic swelling assays.
Analysis of variance revealed that motility before freezing was significantly (P<0.05) more in
MED (68.93 ± 0.5 I %) as compared to CON and HIGH but non-significantly higher than LOW.
Percent post thaw motility (PTM) from semen samples containing MED concentration of CLC
was the highest (61.43 ± 0.74 %; P < 0.05) followed by LOW (50.36 ± 1.15 %; P < 0.05) or
HIGH (38.57 ± 2.66 %; P < 0.05) (Table 1). Percent PTM was the lowest in CON (32.86 ± 2.07
%; P < 0.05) semen samples. Mean plasma membrane integrity (PMI) from semen samples
containing MED concentration of CLC was the highest (59.36 ± 1.32 %; P < 0.05) followed by
LOW (51.93 ± 1.32 %; P < 0.05) or HIGH (44.00 ± 1.65 %; P < 0.05) and was least in CON
(41. I 4 ± 1.71 %; P < 0.05) semen samples. Mean normal acrosomes from semen samples
containing MED concentration of CLC was the highest (52.93 ± 1.78 %; P < 0.05) followed by
HIGH (40.57 ± 2.01 %; P < 0.05) and CON (34.93 ± 2.89 %; P < 0.05) semen samples. Buffalo
bull semen in MED & LOW had significantly (P < 0.05) higher live sperm percentage (69.64 ±
1.84 & 6 1.43 ± 1.62, respectively) as compared to HIGH and CON samples. CON samples had
significantly (P<0.05) higher morphological abnormalities (9.86 ± 0.14 %) as compared to LOW
& MED but non-significantly higher as compared to HIGH samples. All the variables under
study had strong positive correlation (P < 0.01) with each other except morphological
abnormalities having strong negative correlation (P < 0.01) with all others. It is concluded that
addition of cholesterol in MED concentration (4 mg CLCf ml of semen extender) to buffalo bull
semen can improve post-thaw semen quality.
Availability: Items available for loan: UVAS Library [Call number: 1398,T] (1).
32.
Dynamics Of Membrane Changes And Generation Of Reactive Oxygen Species During Bovine Sperm Death
by Mushtaq Ahmad | Prof. Dr. Nasim Ahmad | Dr. Amjad Riaz | Dr. Aneela Zameer Durrani.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1430,T] (1).
33.
Effect Of (Estradiol Benzoate) On Estrus Response And Fertility In Cidr Treated Crossbred Heifers.
by Muhammad Usman Mehmood | Dr. Mian Abdul Sattar | Dr. Muhammad | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Commercial dairy farming in Pakistan is rapidly growing. Crossbred dairy animals hold promise because of two reasons, first that they are significantly high producer than buffalo, Sahiwal, Cholistani and Desi cows and secondly that they are much more adaptable as compared to exotic (Friesian or Jerseys) cows. Generally, replacement heifers remain ignored by the owners in term of feeding and early achievement of puberty as they are not producing. Estradiol promotes tighter synchrony and ovulation and provides opportunity for fixed time A.I as reported earlier in Holstein dairy cows. Use of CIDR has been reported to be effective for synchronization in variety of dairy animals including buffaloes, dairy and beef cows. However no such report has been published on crossbred heifers. The objectives of the present study were to investigate the effect of estradiol benzoate (EB) on estrus intensity, response and fertility in CIDR treated cross bred heifers.Crossbred heifers with average body condition of 3 ± 0.5, located at four farms were assigned throughout one year to receive one of the two treatments. Crossbred heifers with EB (n = 50) or without EB (n = 50). All the heifers were treated with Controlled Internal Drug Releasing Device (CIDR; 1.38 mg Progesterone; Pfizer, USA) on day 0 and were administered injection of PGF2?, (0.150 mg; Dalmazine®, Fatro, Italy; 2 ml; i.m.) on day 6 followed by removal of CIDR on day 7. Estradiol benzoate (400µg/4ml, i.m. Sigma Aldrich Germany) was administered 24 h after the CIDR removal. Estrus detection was carried out by visual observation; twice daily for at least 30 min, beginning after CIDR removal till insemination. All heifers were fixed time inseminated with frozen-thawed semen after 48 and 60 h after CIDR removal, respectively. Pregnancy status was determined by ultrasonography (HS 1500V; Honda; Japan, with 7.5 MHz probe) between days 30 and 40 post AI. Estrus response and pregnancy rate were analyzed using Chi-square test. Pregnancy rate was determined by number pregnant/number treated x 100. The estrus response was 100% in both the groups. The estrus intensity was higher 2.9 ± 0.1in the EB heifers compared to the controls 2.0 ± 0.7. The results showed that pregnancy rate was 54% (27/50) in EB and 36% (18/50) in without EB heifers. The overall pregnancy rate was 45% (45/100). It is concluded that estradiol benzoate in CIDR protocol intensified the signs of estrus without affecting the response, with higher fertility in crossbred heifers.
Availability: Items available for loan: UVAS Library [Call number: 1502,T] (1).
34.
Effect Of Duck Egg Yolk Plasma In Extender On Post Thaw Quality Of Buffalo Bull Spermatozoa
by Furqan Tehseen | Dr. Amjad Riaz | Dr. Hafsa Zaned | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1603,T] (1).
35.
Reproductive and Productive Performance of Dairy Animals Maintained at Mulitary Dairy Farm Lahore Cantt.
by Abrar Ahmed | Dr. Mian Abdul Sattar | Dr. Amjad Riaz | Prof. Dr. Muhammad.
Material type: ; Format:
print
Publisher: 2012Dissertation note: NILI RAVI BUFFALOES
Average age at maturitywas 829.11 ± 30.34 days. Heiferscalved in1999 and 2008 were found to have maximum and minimum age at maturity, respectively. Heifers calved during the winter and dry summer season were found to have maximum and minimum age at maturity, respectively. Mean birth weight of calves was found 28.16±2.8 kg. The effect of birth weight on age at maturity was found to be significant and it was observed that 1 kg increase in birth weight resulted in 16 days decrease in age at maturity. Average age at first conception was 923.37 ± 30.34 days. Heifers calved in 1996 and 2008 were found to have maximum and minimum age at first conception, respectively. Heifers calved during the winter and dry summer season were found to have maximum and minimum age at first conception, respectively. Average age at first calving was 1295.66 ± 62.91 days. Heifers calved in 2002 and 2008 were found to have maximum and minimum age at first calving, respectively. Heifers calved during the dry summer andautumn season were found to have maximum and minimum age at first calving, respectively. Average service period was 164.1 ± 30.15 days. Maximum and minimum service period was in year of 2002 and 2008, respectively. In dry summer and autumn season, service period was maximum and minimum, respectively. In 11th and 10thlactation, service period was maximum and minimum, respectively.Average services per conception were 1.95 ± 0.23. Maximum and minimum services per conception were in 2005 and 2008, respectively. In humid summer and autumn season, services per conception were maximum and minimum respectively. In 11th and 9th lactation, the services per conception were maximum and minimum, respectively. Average gestation period was 310.90 ± 3.07 days. Maximum and minimum gestation periodwas in 2000 and 2009 2009 respectively. In humid summer and dry summer season, gestation period?
was maximum and minimum respectively. In 12th and 8th lactation, the gestation period was maximum and minimum, respectively. Average calving interval was 474.59 ± 29.88 days. Maximum and minimum calving interval was in 2005 and 2001, respectively. In dry summer and autumn season, calving interval was maximum and minimum respectively. In 11th and 10th lactation, calving interval was maximum and minimum, respectively. Average milk yield was 1973.15 ± 53.23 liters. Maximum and minimum milk yield was in 2010 and 2002, respectively. In winter and spring season, milk yield was maximum and minimum respectively. In 4th and 11th lactation, milk yield was maximum and minimum, respectively. Average lactation length was 295.23 ± 64.30 days. Maximum and minimum lactation length was 2001 and 2000, respectively. In spring and humid summer season, lactation length was maximum and minimum respectively. In 10th and 11th lactation, lactation length was maximum and minimum, respectively. Average dry period was 184.93 ± 30.99 days. Maximum and minimum dry period was in 2005 and 2001, respectively. In dry summer and autumn season, dry period was maximum and minimum respectively. In 11th and 10th lactation, dry period was maximum and minimum, respectively.
CROSSBRED COWS
Average age at maturity was 828.33 ± 20.39 days. Heifers calved during the year of 1998 and 1997 were found to have maximum and minimum age at maturity, respectively. Heifers calved during the humid summer and winter season were found to have maximum and minimum age at maturity, respectively. In 15/16 and 1 /2 crossbred level, age at maturity was maximum and minimum, respectively. Mean birth weight of crossbred cow calves was found 23.35 ± 2.8 kg. The effect of birth weight on age at maturity was found to be significant and it was observed that 1 kg increase in birth weight resulted in 07 days decrease in age at maturity. Average age at first conception was 944.18 ± 30.42 days. Heifers calved in 2004 and 1996 were found to have maximum and minimum age at first conception, respectively. Heifers calved during the humid summer and dry summer season were found to have maximum and minimum age at first conception, respectively. In 15/16 and 1 /2 crossbred level, age at first conception was maximum and minimum, respectively. Average age at first calving was 1245.29 ± 40.96 days. Heifers calved in 2008 and 1995 were found to have maximum and minimum age at first calving, respectively. Heifers calved during autumn and winter season were found to have maximum and minimum age at first calving, respectively. In 7/8 and 5/8 crossbred level, age at first calving was maximum and minimum, respectively. Average service period was 252.83 ± 32.56 days. Maximum and minimum service period was in year of 2006 and 2010, respectively. In winter and humid summer season, service period was maximum and minimum respectively. In 10th and 8th lactation, service period was maximum and minimum, respectively. In 7/8 and 15/16 crossbred level, service period was maximum and minimum, respectively. Average services per conception were 2.13 ± 0.31. Maximum and minimum services per conception were in 1999 and 2002, respectively. In winter and dry summer season, services per conception were maximum and minimum respectively. In 9th and 8th lactation, the services per conception were maximum and minimum, respectively. In 15/16 and 5/8 crossbred level, services per conception were maximum and minimum, respectively. Average gestation period was 278.90 ± 4.36 days. Maximum and minimum gestation period was in 2010 and 2003 respectively. In autumn and winter season, gestation period was maximum and minimum, respectively. In 2nd and 7th lactation, the gestation period was maximum and minimum, respectively. In 7/8 and 15/16 crossbred level, gestation period was maximum and minimum, respectively. Average calving interval was 507.03 ± 33.61 days. Maximum and minimum calving interval was in 2006 and 2000, respectively. In winter and humid summer season, calving interval was maximum and minimum respectively. In 11th and 10th lactation, calving interval was maximum and minimum, respectively. In 5/8 and 15/16 crossbred level, calving interval was maximum and minimum, respectively. Average milk yield was 2141.05 ± 90.10 liters. Maximum and minimum milk yield was in 2008 and 2003, respectively. In dry summer and autumn season, milk yield was maximum and minimum respectively. In 11th and 1st lactation, milk yield was maximum and minimum, respectively. In 15/16 and 3/4 crossbred level, milk yield was maximum and minimum, respectively. Average lactation length was279.31 ± 8.33 days. Maximum and minimum lactation length was 2001 and 2003, respectively. In dry summer and autumn season, lactation length was maximum and minimum respectively. In 11th and 5th lactation, lactation length was maximum and minimum, respectively. In 3/4 and 15/16 crossbred level, lactation length was maximum and minimum, respectively. Average dry period was 238.64 ± 33.93 days. Maximum and minimum dry period was in 2006 and 2000, respectively. In winter and humid summer season, dry period was maximum and minimum respectively. In 5th and 11th lactation, dry period was maximum and minimum, respectively. In 5/8 and 15/16 crossbred level, dry period was maximum and minimum, respectively.
Availability: Items available for loan: UVAS Library [Call number: 1624,T] (1).
36.
Effect of Equine Chorionic Gonadotropin (eCG) on the Follicular Dynamics, Estrus Eepression, Ovulation and Pregnancy Rate in Control Internal Drug Release (CIDR) Based Estrus Synchronization Protocol in NIli-Ravi Buffalo.
by Muhammad Ilyas Naveed | Prof. Dr. Nasim Ahmad | Dr. Amjad Riaz | Prof. Dr. Muhammad.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Buffalo is unique to livestock and dairy industry in Pakistan as its share is major (65%) in milk production major. Reproduction is crucial for profitable production in dairy sector. Buffalo reproduction is hampered due to small size ovaries, poor ovarian reserves, and less pronounced estrus intensity, ultimately leading to substandard fertility. Synchronization technology including CIDR based protocols are well established in cows and gaining popularity in buffaloes. However, this needs modifications based on estrous cycle physiology in buffaloes. It is hypothesized that addition of eCG at the time inducing luteolysis in CIDR based synchronization protocol will promote follicular growth, ovulation and pregnancy rates. The objective of this study is to determine the effect of addition of eCG to a CIDR based synchronization protocol on ovarian follicular dynamics, estrus rate, ovulation, and pregnancy rates in Nili-Ravi buffalo. Lactating multiparous suckled Nili-Ravi buffalo (n=63) milked twice daily were assigned in a CIDR (1.38 g progesterone Pfizer Co, USA) synchronization regimen. Animals were divided randomly to receive either saline (n=31) or 1000 IU eCG i.m. (n=32) (Chronogest PMSG, Intervet, Holland) concurrent with PGF2á (Dalmazine, cloprostenol, Fatro, Italy) treatment of the CIDR protocol (Day 6). Fixed time, two inseminations were performed at 48 and 60 hours after CIDR removal (Day 7). Number of follicles of variable size recruited at CIDR removal, dominant follicle size, its growth rate and ovulation time did not differ significantly between the groups. The estrus response was not higher significantly (P>0.14) in eCG group than control group but estrus intensity was significantly higher (P>0.001) in eCG group. Ovulation rate and 26 pregnancy per AI was higher (P>0.05 & 0.004) in buffaloes in eCG group. Therefore, It is concluded that eCG is helpful to improve estrus expression, ovulation rate and pregnancy per AI in FTAI program in Nili-Ravi buffaloes.
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37.
Effect Of Mineral Variation In Soil, Water, Fodder And Serum On Reproductive Efficiency Of Buffaloes
by Muhammad Binyameen | Dr. Mian Abdul Satter | Prof. Dr. Khalid | Prof. Dr. Nasim ahmed.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: This was the first study in which maximum parameters were covered to see the effect of mineral variation in canal water, drinking water, soil and fodder on blood serum. This study was conducted at canal water, drinking water, soil, fodder (Berseem), and blood samples from different groups of buffaloes at Livestock Experiment Station Bhunikey Distt. Kasur. The objectives of these samplings were to find out the concentration of calcium, magnesium, and inorganic phosphorus in the different types of samples and also to find out the effect of mineral variation on reproductive efficiency of buffaloes.
All the sample collection was carried out on standard basis. Analyses of water and soil were performed at Agriculture Soil and Fertility Center (Thokar Niaz Baig) Lahore. To overcome variations, water sample used for drinking of buffaloes and for irrigation of soil were collected and checked separately. Soil samples were taken from same places at different depths from where fodder was coming. Samples of soil were collected between 0-18 inches up to where roots of fodder can penetrate. The soil was highly deficient in phosphorus. Fodder (Berseem) samples were checked at the start of December and at the end of March. The values of minerals were significantly (P < 0.05) low in fodder (Berseem) at the end of March because Trifolium alxendrium was at ripening stage. The history of animal reproductive status was confirmed by Honda ultrasound at 2.5 Mega Hertz. Serum minerals profile was analyzed at Buffalo Research Institute (Pattoki). All the animals were lactating excluding heifers. Blood serum of all buffaloes in this study was analyzed twice at the interval of two months to check the variation. The levels of minerals were non-significant (P > 0.05) among the groups but were significant (P < 0.05) between the groups at the start of December and at the end of March. Overall calcium was low in anestrus heifers. The calcium level was in blood serum low at the end of March.
The level of magnesium were significantly (P < 0.05) different between canal and drinking water. Magnesium was non-significant (P > 0.05) in soil at different depths and similarly in fodder (Berseem) at start of December and at the end of March. Magnesium was significantly (P < 0.05) different in blood serum at start and end of March between the groups. Level of magnesium was low in normal cycling and repeat breeder buffaloes. Inorganic phosphorus was non-significant (P > 0.05) among water, soil, fodder (Berseem) and blood serum and concentration of inorganic phosphorus was low in non cycling buffaloes. Deficiency of minerals in soil, and drinking water was significantly (P < 0.05) contributing its effect on fodder. Anestrus heifers had lower Ca: P ratio among the buffaloes groups.
All the results were analyzed through Independent sample T test. To observe the relationship between mineral profile of soil, water, fodder and serum of buffaloes Correlation Coefficient and multiple regression was applied. To compare difference between mineral profiles of 4 groups in buffaloes serum ANOVA was applied (Steel et al, 1997). Difference between means compared through DMR (Duncan. 1955) by using SPSS version 13. It was concluded from this study inorganic phosphorus is required to soil of Distt. Kasur. Mineral mixture rich in calcium required to anestrus heifers and Ca: P ratio was low in this group.
Availability: Items available for loan: UVAS Library [Call number: 1658,T] (1).
38.
Effect of Timing of Artificial Insemination and Ovulation in Relation to Onset of Standing Heat on Pregnancy Rate in Nili-Ravi Buffaloes
by Umair Riaz | Prof. Dr. Nasim Ahmad | Dr. Mian Abdul Sattar | Prof. Dr.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Artificial insemination (AI) is one of the first and most influential biotechnologies of animal agriculture. To be beneficial artificial insemination must be done properly. There are many factors involved in quality of AI performance, including insemination technique, technician, semen quality, cleanliness, etc. However, the single most important factor in achieving high conception rate in buffalo is the timing of AI relative to the timing of ovulation. Application of AI is difficult because of poor estrus expression by buffaloes and poor estrus detection by owners, a highly variable duration of estrus and the difficulty to predict time of ovulation. The objective of the present study was to determine the effect of A.I at different time intervals (0, 12, 24 hours after the onset of standing heat) in relation to time of ovulation on pregnancy rate in buffalo. For this purpose, buffaloes with average body condition 2.5 ± 0.5 at Livestock Experimental Station Bhuneki, Buffalo Research Institute, Pattoki District Kasur during breeding season (September to December) were exposed to teaser bull two times in a day at 12 hours interval (6:00 am in morning and 6:00 pm in evening) to determine the standing heat (0h). Buffaloes, allocated randomly, at different time intervals i.e., either 0, 12 or 24 hours after the onset of standing heat were bred artificially using frozen thawed semen. Trans-rectal ultrasonography (HS 1500V; Honda; Japan, with 7.5 MHz transducer) was used to determine ovarian follicular development and ovulation, from onset of standing heat till ovulation after every 12 hours. Pregnancy status was determined by ultrasonography, 30 to 40 days post AI. Results revealed that the overall time of ovulation from onset of standing heat averaged 35.33 ± 0.21 h. Pregnancy rates were 26% (8/30) at 0 h, 37% (10/27) at 12 h and 53% (15/28) at 24 h after the onset of standing heat. It is concluded that timing of ovulation is about 35 h and 24
maximum pregnancy rate are achieved when bred after 24 hours after the onset of heat in buffaloes.
Availability: Items available for loan: UVAS Library [Call number: 1694,T] (1).
39.
Effect Of Control Internal Drug Release & Human Chorionic Gonadotropin Administration On Day 7 After Fixed Time
by Ali Husnain | Prof. Dr. Nasim Ahmad | Dr Mian Abdul sattar | Prof. Dr.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1697,T] (1).
40.
Effect Of Butylated Hydroxytoluene (Bht) As Antioxidant On Semen Quality Of Naked Neck Chicken
by Waseem Abbass | Dr. Amjad Riaz | Prof. Dr | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1746,T] (1).
41.
Comparision Of Synchronization Protocols On Estrus, Follicular Dynamics, Ovulation Time And Conception Rate in Sahiwal Cows
by Mubbashar Hassan | Prof. Dr. Nasim Ahmad | Dr. Aijaz Ali Channa | Dr. Aneela.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Sahiwalcow is one of the established milch breed of zebu cattle (Bosindicus), known for heat tolerance and tick resistance in tropical and subtropical region which has its origin in Pakistan.Average milk yield is about 1500-2200 liters per lactation with 4.5% contents of fat (Shah, 1994).The population of Sahiwal cattle in Pakistan is decreasing over the past few decades due to change in agriculture system, indiscriminate crossbreeding and preference for buffalo. To minimize the cross breeding of Sahiwal cows, efforts have been made for its conservation, through management and genetic improvement. Significant genetic improvement, through artificial insemination(A.I) has been made. Fixed time artificial insemination (FTAI) has emerged in recent times which usually performed in association with a variety of estrus and ovulation synchronization protocols.Synchronization of estrus is actually the manipulation of estrous cycle or induction of estrus to bring large group of females in estrus at a programmed time. Three synchronization protocols were compared. In PG group, each animal was treated with luteolytic dose of PGF2? (d-Cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m) on random stage of the estrous cycle. In OVS group each cow received an intramuscular injection of gonadotropin releasing hormone (GnRH; 50 mcg of a GnRH analogue, DalmarelinTMFatro®, Italy; 2 ml; i.m) on random stage of estrus cycle (day 0). On day 7 these cows were treated with PGF2? (d- cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m), followed by second injection of GnRH on day 9. In CIDR group, each animal received an intravaginal Controlled Internal Drug Releasing Device (CIDR™; 1.38 g progesterone Eazibreed™; InterAg, Hamilton, New Zealand) on random stage of estrus cycle that was considered as day 0 of treatment. CIDR was removed on day 7 and on day 6 cows were injected with luteolytic dose of PGF2? (d-cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m).Estrus response and estrus intensity are higher in CIDR group but ovulation rate is less in CIDR and high in PG and OVS group. Timing of ovulation is shortest in OVS group as compared to other groups. Pregnancy rate are also high in OVS group. Therefore it is concluded that Ovsynch protocol is helpful to improve ovulation and pregnancy rate in Sahiwal cows.
Availability: Items available for loan: UVAS Library [Call number: 1827,T] (1).
42.
Effeect Of Ascorbic Acid And Moulting On Semen Quality In Native Chickens
by Adnan Jabbar | Dr Amjad Riaz | Prof Dr | Prof Dr Mian Abdual Sattar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1860,T] (1).
43.
Effect Of Cholesterol On Post Thaw Semen Quality And Fertility In Sahiwal Bulls
by Ahmed Gul Tarin | Dr Mian Abdual Sattar | Dr Khurshid Ahmed | Prof Dr Khalid | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1861,T] (1).
44.
A Study On Superovuation Protocol For The Development Of Embryo Transfer Technique In Mice
by Muhammad Ameen jamal | Dr. Amjad riaz | Dr. Aamir | Prof. Dr Mian abdul sattar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1900,T] (1).
45.
Effect Of Trehalose On Cryopreservation Of Sahiwal Bull Spermatozoa
by Zohaib Ashraf | Dr. Aijaz ali channa | Prof .Dr. Aneela | Prof. DR. Nasim ahmad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1948,T] (1).
46.
Effect Of Cholesterol Loaded Cyclodextrins (Clc) On Post-Thaw Quality In Canine Spermatozoa
by Junaid khan | Prof. Dr.Nasim ahmad | Dr. Asim khalid | Prof. Dr. Mian abdul sattar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2005,T] (1).
47.
Standardization Of Bovine Serum Albumin (Bsa) For Cryopreservation Of Beetal Buck Semen
by Muhammad Shahzad | Prof. Dr. Mian abdul sattar | Prof. Dr | Prof. Dr. Naseem ahmad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2018,T] (1).
48.
Effect Of Rbst Treatment On Antral Follicular Count Plasma E2 P4 Profile And Esteus Behavior In Postpartum Nili Ravi Buffaloes
by Sadia naz | Dr. Amjad riaz | Dr. Jawad nazir | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2055,T] (1).
49.
Effect Of Cholesterol-Loaded Cyclodextrin (Clc) Addition On Egg Yolk Ratio In Semen Extender And Post-Thaw Quality Of Buffalo Bull Sperm
by Mehboob Ahmed | Dr. Mushtaq Ahmad | Prof. Dr. Mian Abdul Sattar.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2085,T] (1).
50.
Enhancing Fertility Through Induction Of Ovulation In Mares
by Fayzan sultan awan | Dr. Muhammad Usman Mehmood | Prof. Dr. Aneela | Prof. Dr. Nasim Ahmad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2161,T] (1).
